Determination of aspartate kinase in maize tissues.

Ferreira, Renato Rodrigues and Vendemiatti, Ariane and Gratão, Priscila Lupino and Lea, Peter J. and Azevedo, Ricardo Antunes (2005) Determination of aspartate kinase in maize tissues. Scientia Agricola, 62 (2). pp. 184-189. ISSN 1678-992X

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Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity.

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Journal Article
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Scientia Agricola
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21 Jul 2008 13:07
Last Modified:
21 Nov 2022 17:59