DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts

Allinson, Sarah L. and Dianova, Irina I. and Dianov, Grigory L. (2001) DNA polymerase B is the major dRP lyase involved in repair of oxidative base lesions in DNA by mammalian cell extracts. EMBO Journal, 20 (23). pp. 6919-6926. ISSN 0261-4189

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Abstract

The repair of oxidative base lesions in DNA is a coordinated chain of reactions that includes removal of the damaged base, incision of the phosphodiester backbone at the abasic sugar residue, incorporation of an undamaged nucleotide and sealing of the DNA strand break. Although removal of a damaged base in mammalian cells is initiated primarily by a damage-specific DNA glycosylase, several lyases and DNA polymerases may contribute to the later stages of repair. DNA polymerase (Pol ) was implicated recently as the major polymerase involved in repair of oxidative base lesions; however, the identity of the lyase participating in the repair of oxidative lesions is unclear. We studied the mechanism by which mammalian cell extracts process DNA substrates containing a single 8-oxoguanine or 5,6-dihydrouracil at a defined position. We find that, when repair synthesis proceeds through a Pol -dependent single nucleotide replacement mechanism, the 5'-deoxyribosephosphate lyase activity of Pol is essential for repair of both lesions.

Item Type:
Journal Article
Journal or Publication Title:
EMBO Journal
Uncontrolled Keywords:
/dk/atira/pure/researchoutput/libraryofcongress/qh301
Subjects:
?? BASE EXCISION REPAIRCELL EXTRACTSDNA POLYMERASENTH1OGG1NEUROSCIENCE(ALL)BIOCHEMISTRY, GENETICS AND MOLECULAR BIOLOGY(ALL)MOLECULAR BIOLOGYIMMUNOLOGY AND MICROBIOLOGY(ALL)QH301 BIOLOGY ??
ID Code:
10164
Deposited By:
Deposited On:
08 Jul 2008 10:58
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Sep 2023 00:05