Diggle, Stephen P. and Lumjiaktase, Putthapoom and Dipilato, Francesca and Winzer, Klaus and Kunakorn, Mongkol and Barrett, David A. and Ram Chhabra, Siri and Cámara, Miguel and Williams, Paul (2006) Functional Genetic Analysis Reveals a 2-Alkyl-4-Quinolone Signaling System in the Human Pathogen Burkholderia pseudomallei and Related Bacteria. Chemistry & Biology, 13 (7). pp. 701-710. ISSN 1074-5521Full text not available from this repository.
Pseudomonas aeruginosa synthesizes diverse 2-alkyl-4(1H)-quinolones (AHQs), including the signaling molecule 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS), via the pqsABCDE locus. By examining the genome databases, homologs of the pqs genes were identified in other bacteria. However, apart from P. aeruginosa, only Burkholderia pseudomallei and B. thailandensis contained a complete pqsA–E operon (termed hhqA–E). By introducing the B. pseudomallei hhqA and hhqE genes into P. aeruginosa pqsA and pqsE mutants, we show that they are functionally conserved and restore virulence factor and PQS production. B. pseudomallei, B. thailandensis, B. cenocepacia, and P. putida each produced 2-heptyl-4(1H)-quinolone (HHQ), but not PQS. Mutation of hhqA in B. pseudomallei resulted in the loss of AHQ production, altered colony morphology, and enhanced elastase production, which was reduced to parental levels by exogenous HHQ. These data reveal a role for AHQs in bacterial cell-to-cell communication beyond that seen in P. aeruginosa.
|Journal or Publication Title:||Chemistry & Biology|
|Uncontrolled Keywords:||MICROBIO ; SIGNALING ; CHEMBIO|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
|Deposited By:||Dr Klaus Winzer|
|Deposited On:||13 Jun 2008 14:17|
|Last Modified:||26 Jul 2012 18:38|
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