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The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity.

Peiter, Edgar and Fischer, Marc and Sidaway, Kate and Roberts, Stephen K. and Sanders, Dale (2005) The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity. FEBS Letters, 579 (25). pp. 5697-5703. ISSN 0014-5793

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Abstract

Cch1p and Mid1p are components of a high-affinity Ca2+-permeable channel in the yeast plasma membrane. Here, we show that growth of mutants in the Cch1pMid1p channel is markedly hypersensitive to low temperature and to high iron concentration in the medium. Both phenotypes were suppressed by high Ca2+ concentration. Iron stress elicited an increased Ca2+ influx into both wild type and cch1Δmid1Δ yeast. Inhibition of calcineurin strongly depressed growth of iron-stressed wild type yeast, indicating that calcineurin is a downstream element of the iron stress response. Iron hypersensitivity of the cch1Δmid1Δ mutant was not associated with an increased iron uptake. An involvement of oxidative stress in the iron-hypersensitive phenotype was indicated by the findings that the antioxidants tocopheryl acetate and (ethyl)glutathione improved growth and viability of the iron-stressed mutant. Further, the degree of glutathione oxidation was increased in the presence of iron. The results indicate that iron stress leads to an increased oxidative poise and that Cch1pMid1p is essential to tolerate this condition.

Item Type: Article
Journal or Publication Title: FEBS Letters
Uncontrolled Keywords: Calcium ; Channel ; Cold stress ; Iron toxicity ; Saccharomyces cerevisiae
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 9498
Deposited By: Dr Stephen K Roberts
Deposited On: 12 Jun 2008 11:50
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:38
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9498

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