Lancien, M. and McCabe, T. C. and Chang, C. and Roberts, Michael R. (2005) Roles for plant 14-3-3 proteins in signalling and development. Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 141 (3 Supp). S258-S259. ISSN 1095-6433Full text not available from this repository.
14-3-3 Proteins are a class of regulatory protein that affect the activity of a wide range of targets by direct protein–protein interaction. In plants such as Arabidopsis and tomato, 14-3-3s are encoded by a family of around 12–13 genes. With multiple 14-3-3 isoforms and many more target proteins, it is difficult to assign specific biological functions to 14-3-3 proteins. Here, we describe two different approaches that identify roles for 14-3-3 proteins in signalling and plant development. Firstly, we have shown that 14-3-3 proteins are required for pollination in tobacco, by disrupting the function of pollen 14-3-3 proteins via two different mechanisms. Firstly, we used RNAi to silence the most abundant tobacco pollen 14-3-3 gene, and secondly, we used a pollen-specific promoter to express a generic 14-3-3 inhibitor protein. Pollen with reduced 14-3-3 activity produced by both methods demonstrated a reduced ability to fertilise tobacco ovules. Secondly, we have identified the Arabidopsis ethylene signalling protein, CTR1, as a 14-3-3-interacting protein. We find that mutation of a 14-3-3 binding site in CTR1 blocks its activity when introduced into transgenic plants, resulting in constitutive ethylene signalling and aberrant plant development. These data clearly indicate a requirement for the CTR1-14-3-3 interaction for normal ethylene signalling in Arabidopsis.
|Journal or Publication Title:||Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology|
|Uncontrolled Keywords:||14-3-3 Proteins ; Pollen ; Ethylene ; CTR1|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Dr Michael R Roberts|
|Deposited On:||12 Jun 2008 11:50|
|Last Modified:||19 Apr 2016 00:17|
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