Lancien, M. and McCabe, T. C. and Chang, C. and Roberts, Michael R. (2005) Roles for plant 14-3-3 proteins in signalling and development. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 141 (3 Supp). S258-S259. ISSN 1095-6433
Full text not available from this repository.Abstract
14-3-3 Proteins are a class of regulatory protein that affect the activity of a wide range of targets by direct protein–protein interaction. In plants such as Arabidopsis and tomato, 14-3-3s are encoded by a family of around 12–13 genes. With multiple 14-3-3 isoforms and many more target proteins, it is difficult to assign specific biological functions to 14-3-3 proteins. Here, we describe two different approaches that identify roles for 14-3-3 proteins in signalling and plant development. Firstly, we have shown that 14-3-3 proteins are required for pollination in tobacco, by disrupting the function of pollen 14-3-3 proteins via two different mechanisms. Firstly, we used RNAi to silence the most abundant tobacco pollen 14-3-3 gene, and secondly, we used a pollen-specific promoter to express a generic 14-3-3 inhibitor protein. Pollen with reduced 14-3-3 activity produced by both methods demonstrated a reduced ability to fertilise tobacco ovules. Secondly, we have identified the Arabidopsis ethylene signalling protein, CTR1, as a 14-3-3-interacting protein. We find that mutation of a 14-3-3 binding site in CTR1 blocks its activity when introduced into transgenic plants, resulting in constitutive ethylene signalling and aberrant plant development. These data clearly indicate a requirement for the CTR1-14-3-3 interaction for normal ethylene signalling in Arabidopsis.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology |
| Uncontrolled Keywords: | 14-3-3 Proteins ; Pollen ; Ethylene ; CTR1 |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 9496 |
| Deposited By: | Dr Michael R Roberts |
| Deposited On: | 12 Jun 2008 11:50 |
| Refereed?: | No |
| Published?: | Published |
| Last Modified: | 26 Jul 2012 18:37 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/9496 |
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