Holtman, W. L. and Roberts, Michael R. and Wang, Mei (2000) 14-3-3 proteins and a 13-lipoxygenase form associations in a phosphorylation-dependent manner. Biochemical Society Transactions, 28 (6). pp. 834-836. ISSN 0300-5127
Full text not available from this repository.Abstract
Recently, we have demonstrated by two different methods that lipoxgenases (LOXs) and 14-3-3 proteins form interactions in barley embryos [Holtman, Roberts, Oppedijk, Testerink, van Zeijl and Wang (2000) FEBS Lett. 474, 48–52]. It was shown by both co-immunoprecipitations and surface-plasmon resonance experiments that 13-LOX, but not 9-LOX, forms interactions with 14-3-3 proteins. In the present report we show that the presence of 13-LOX and 14-3-3 proteins was established in high-molecular-mass complexes. Amounts of 13-LOX and 14-3-3 proteins in high-molecular-mass fractions increased during germination, but were reduced after dephosphorylation of protein extracts or competition with the 14-3-3-binding peptide P-Raf-259, indicating that 13-LOX and 14-3-3 proteins interact in a phosphorylation-dependent manner.
| Item Type: | Article |
|---|---|
| Journal or Publication Title: | Biochemical Society Transactions |
| Uncontrolled Keywords: | lipid metabolism ; protein interactions. |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Departments: | Faculty of Science and Technology > Lancaster Environment Centre |
| ID Code: | 9487 |
| Deposited By: | Dr Michael R Roberts |
| Deposited On: | 11 Jun 2008 16:47 |
| Refereed?: | Yes |
| Published?: | Published |
| Last Modified: | 24 Sep 2012 16:56 |
| Identification Number: | |
| URI: | http://eprints.lancs.ac.uk/id/eprint/9487 |
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