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Defective cell cycle control underlies abnormal cortical development in the hydrocephalic Texas rat.

Owen-Lynch, P. Jane and Draper, C. E. and Mashayekhi, F. and Bannister, C. M. and Miyan, J. A. (2003) Defective cell cycle control underlies abnormal cortical development in the hydrocephalic Texas rat. Brain, 126 (3). pp. 623-631. ISSN 1460-2156

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Abstract

There is a significant body of evidence to suggest a physiological role for the CSF in both the developing and adult brain. Our recent studies suggest a critical role for this fluid in the developing brain of the hydrocephalic Texas (H-Tx) rat. A key feature of the foetal-onset hydrocephalus in this rat is obstruction in the flow and/or absorption of fluid that is associated with abnormal development of the cerebral cortex resulting in a reduction in the number of neuronal precursors generated. Cells from the affected cerebral cortex do proliferate in vitro and show dose-dependent responses to growth factor stimulation, suggesting that germinal cells are under inhibitory influences in vivo. We tested the hypothesis that the CSF of the affected brains was responsible for the abnormal development. Cells analysed at the time of extraction from affected brains showed an accumulation of cells in the S-phase of the cell cycle, which was reflected in a concentration of cells containing high levels of DNA in the germinal matrix of histological sections of affected brains. CSF from the lateral ventricle of affected foetal brains not only inhibited in vitro proliferation of normal neuronal progenitors, but it also resulted in an accumulation of cells in the S-phase of the cell cycle mimicking the situation in vivo. Fluid from normal foetal brains did not have this effect. From the work detailed here on the mechanistic basis of the deficient cortical development in the foetal hydrocephalic rat brain, we conclude that the content of the CSF is critical in maintaining germinal matrix function and output and, therefore, that the CSF has a vital role in brain development.

Item Type: Article
Journal or Publication Title: Brain
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 9260
Deposited By: Dr P Jane Owen-Lynch
Deposited On: 03 Jun 2008 09:55
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:34
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9260

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