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Characterisation of a differentially expressed protein that shows an unusual localisation to intracellular membranes in Leishmania major.

Kneupfer, Ellen and Stierhof, Y. D. and McKean, Paul G. and Smith, Deborah F. (2001) Characterisation of a differentially expressed protein that shows an unusual localisation to intracellular membranes in Leishmania major. Biochemical Journal, 356 (2). pp. 335-344. ISSN 0264-6021

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Abstract

The SHERP genes are found as a tandem pair within the differentially regulated LmcDNA16 locus of Leishmania major. The SHERP gene product (Small Hydrophilic Endoplasmic Reticulum-associated Protein) is unusual in its small size (6.2kDa), its acidic pI (4.6) and its exclusive, high-level expression ( 100000 copies per cell) in infective non-replicative parasite stages. No homologues have been found to date. Secondary-structure predictions suggest that SHERP contains an amphiphilic a-helix that is presumably involved in protein–protein interactions. SHERP has been localized to the endoplasmic reticulum as well as to the outer mitochondrial membrane in both wild-type and over-expressing parasites. Given the absence of an N-terminal signal sequence, transmembrane-spanning domains or detectable post-translational modifications, it is likely that this hydrophilic molecule is a peripheral membrane protein on the cytosolic face of intracellular membranes. This weak membrane association has been confirmed in cell-fractionation assays, in which SHERP redistributes from the cytoplasmic to the membrane fraction after in vivo cross-linking. SHERP does not appear to be involved in rearrangements of the cytoskeleton or conservation of organelle morphology during parasite differentiation. The role of this novel protein, presumed to be part of a protein complex, in infective parasites that are nutrient-deficient and pre-adapted for intracellular survival in the mammalian host is under investigation.

Item Type: Article
Journal or Publication Title: Biochemical Journal
Uncontrolled Keywords: differential gene expression ; endoplasmic reticulum ; metacyclic ; mitochondrial protein.
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 9245
Deposited By: Dr Paul G McKean
Deposited On: 02 Jun 2008 13:47
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:34
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9245

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