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The flagella connector of Trypanosoma brucei : an unusual mobile transmembrane junction.

Briggs, Laura and McKean, Paul G. and Baines, Andrea and Moreira-Leite, Flavia and Davidge, Jacqueline and Vaughan, Sue and Gull, Keith (2004) The flagella connector of Trypanosoma brucei : an unusual mobile transmembrane junction. Journal of Cell Science, 117 (9). pp. 1641-1651. ISSN 0021-9533

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    Abstract

    Throughout its elongation, the new flagellum of the procyclic form of the African trypanosome Trypanosoma brucei is tethered at its tip to the lateral aspect of the old flagellum. This phenomenon provides a cytotactic mechanism for influencing inheritance of cellular pattern. Here, we show that this tethering is produced via a discrete, mobile transmembrane junction – the flagella connector. Light and electron microscopy reveal that the flagella connector links the extending microtubules at the tip of the new flagellum to the lateral aspect of three of the doublet microtubules in the old flagellar axoneme. Two sets of filaments connect the microtubules to three plates on the inner faces of the old and new flagellar membranes. Three differentiated areas of old and new flagellar membranes are then juxtaposed and connected by a central interstitial core of electron-dense material. The flagella connector is formed early in flagellum extension and is removed at the end of cytokinesis, but the exact timing of the latter event is slightly variable. The flagella connector represents a novel form of cellular junction that is both dynamic and mobile.

    Item Type: Article
    Journal or Publication Title: Journal of Cell Science
    Uncontrolled Keywords: Trypanosome ; Flagellum ; Morphogenesis ; Cell junction ; Flagella connector ; Cytotaxis
    Subjects: Q Science > QH Natural history > QH301 Biology
    Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
    ID Code: 9236
    Deposited By: Dr Paul G McKean
    Deposited On: 02 Jun 2008 11:01
    Refereed?: Yes
    Published?: Published
    Last Modified: 26 Jul 2012 18:34
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/9236

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