Lancaster EPrints

Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes.

Gadelha, C. and Wickstead, B. and McKean, P. G. and Gull, K. (2006) Basal body and flagellum mutants reveal a rotational constraint of the central pair microtubules in the axonemes of trypanosomes. Journal of Cell Science, 119 (12). pp. 2405-2413. ISSN 0021-9533

[img]
Preview
PDF (2405.pdf)
Download (1025Kb) | Preview

    Abstract

    Productive beating of eukaryotic flagella and cilia requires a strict regulation of axonemal dynein activation. Fundamental to any description of axonemal beating is an understanding of the significance of the central pair microtubules and the degree to which central pair rotation has a role. However, for the majority of organisms, it is unclear whether the central pair actually rotates. Using an extra-axonemal structure as a fixed reference, we analysed the orientation of the central pair in African trypanosomes and other kinetoplastid protozoa. A geometric correction allowed the superposition of data from many cross-sections, demonstrating that the axis of the central pair is invariant and that there is no central pair rotation in these organisms. Analysis of mutants depleted in particular flagellar and basal body proteins [-tubulin, -tubulin, Parkin co-regulated gene product (PACRG) or the paraflagellar rod protein PFR2] allowed a dissection of the mechanisms for central pair constraint. This demonstrated that orientation is independent of flagellum attachment and beating, but is influenced by constraints along its length and is entirely dependent on correct positioning at the basal plate.

    Item Type: Article
    Journal or Publication Title: Journal of Cell Science
    Uncontrolled Keywords: Axoneme ; Flagellum ; Central pair ; Paraflagellar rod ; Delta-tubulin ; Basal body
    Subjects: Q Science > QH Natural history > QH301 Biology
    Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
    ID Code: 9232
    Deposited By: Dr Paul G McKean
    Deposited On: 02 Jun 2008 11:01
    Refereed?: Yes
    Published?: Published
    Last Modified: 26 Jul 2012 18:33
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/9232

    Actions (login required)

    View Item