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Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation.

Davidge, Jacqueline A. and Chambers, Emma and Dickinson, Harriet A. and Towers, Katie and Ginger, Michael L. and McKean, Paul G. and Gull, Keith (2006) Trypanosome IFT mutants provide insight into the motor location for mobility of the flagella connector and flagella membrane formation. Journal of Cell Science, 119 (19). pp. 3935-3943. ISSN 0021-9533

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    Abstract

    The flagella connector (FC) of procyclic trypanosomes is a mobile, transmembrane junction important in providing cytotactic morphogenetic information to the daughter cell. Quantitative analyses of FC positioning along the old flagellum, involving direct observations and use of the MPM2 anti-phosphoprotein monoclonal reveals a `stop point' is reached on the old flagellum which correlates well with the initiation of basal body migration and kinetoplast segregation. This demonstrates further complexities of the FC and its movement in morphogenetic events in trypanosomes than have hitherto been described. We used intraflagellar transport RNAi mutants to ablate the formation of a new flagellum. Intriguingly the FC could still move, indicating that a motor function beyond the new flagellum is sufficient to move it. When such a FC moves, it drags a sleeve of new flagellar membrane out of the flagellar pocket. This axoneme-less flagellar membrane maintains appropriate developmental relationships to the cell body including following the correct helical path and being connected to the internal cytoskeleton by macula adherens junctions. Movement of the FC in the apparent absence of intraflagellar transport raises the possibility of a new form of motility within a eukaryotic flagellum.

    Item Type: Article
    Journal or Publication Title: Journal of Cell Science
    Uncontrolled Keywords: Trypanosome ; Morphogenesis ; Flagellar connector ; Phosphorylation ; Basal body
    Subjects: Q Science > QH Natural history > QH301 Biology
    Departments: Faculty of Science and Technology > Lancaster Environment Centre
    Faculty of Health and Medicine > Biomedical & Life Sciences
    ID Code: 9229
    Deposited By: Dr Paul G McKean
    Deposited On: 02 Jun 2008 11:02
    Refereed?: Yes
    Published?: Published
    Last Modified: 26 Jul 2012 18:33
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/9229

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