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The proteolytic release of gentoxins from cooked beef.

Martin, Francis L. and Cole, K. J. and Phillips, D. H. and Grover, Philip L. (2002) The proteolytic release of gentoxins from cooked beef. Biochemical and Biophysical Research Communications, 293 (5). pp. 1497-1501. ISSN 0006-291X

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Abstract

Dietary factors are important in the aetiology of human cancer and carcinogens, mostly heterocyclic aromatic amines, have been isolated from cooked proteinaceous foodstuffs. Whilst such carcinogens have induced tumours in rodent bioassays, the dosages required were much higher than estimates of human exposure levels. We have examined the possibility that genotoxins, which were not extractable prior to enzymic digestion, may be released from cooked beef by proteolysis. Dichloromethane and/or a solid-phase tandem extraction procedure were used with aqueous homogenates of pan-fried or uncooked beef, both before and after proteolysis (proteinase K). Genotoxicity was measured using the alkaline single cell–gel electrophoresis (‘Comet') assay in MCL-5 cells and mutagenicity in Salmonella typhimurium strains TA1538 or YG1019. Proteolysis released significant amounts of DNA-damaging material that was not extractible prior to enzymic digestion, suggesting that human exposures to diet-derived genotoxins may have been underestimated.

Item Type: Article
Journal or Publication Title: Biochemical and Biophysical Research Communications
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 9201
Deposited By: Dr Francis L Martin
Deposited On: 30 May 2008 13:48
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:33
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9201

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