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Use of a highly sensitive two-dimensional luminescence imaging system to monitor endogenous bioluminescence in plant leaves.

Flor-Henry, Michel and McCabe, Tulene C and De Bruxelles, Guy L. and Roberts, Michael R (2004) Use of a highly sensitive two-dimensional luminescence imaging system to monitor endogenous bioluminescence in plant leaves. BMC Plant Biology, 4 (9).

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    Abstract

    Background All living organisms emit spontaneous low-level bioluminescence, which can be increased in response to stress. Methods for imaging this ultra-weak luminescence have previously been limited by the sensitivity of the detection systems used. Results We developed a novel configuration of a cooled charge-coupled device (CCD) for 2-dimensional imaging of light emission from biological material. In this study, we imaged photon emission from plant leaves. The equipment allowed short integration times for image acquisition, providing high resolution spatial and temporal information on bioluminescence. We were able to carry out time course imaging of both delayed chlorophyll fluorescence from whole leaves, and of low level wound-induced luminescence that we showed to be localised to sites of tissue damage. We found that wound-induced luminescence was chlorophyll-dependent and was enhanced at higher temperatures. Conclusions The data gathered on plant bioluminescence illustrate that the equipment described here represents an improvement in 2-dimensional luminescence imaging technology. Using this system, we identify chlorophyll as the origin of wound-induced luminescence from leaves.

    Item Type: Article
    Journal or Publication Title: BMC Plant Biology
    Uncontrolled Keywords: bioluminescence plants wounding stress
    Subjects: Q Science > QH Natural history > QH301 Biology
    Departments: Faculty of Arts & Social Sciences > Linguistics & English Language
    Faculty of Science and Technology > Lancaster Environment Centre
    ID Code: 91
    Deposited By: Dr Michael R Roberts
    Deposited On: 12 Oct 2005
    Refereed?: Yes
    Published?: Published
    Last Modified: 06 Sep 2013 21:18
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/91

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