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Tight junction-related protein expression and distribution in human corneal epithelium.

Ban, Yuriko and Dota, Atsuyoshi and Cooper, Leanne J. and Fullwood, Nigel J. and Nakamura, Takahiro and Tsuzuki, Masakatsu and Mochida, Chikako and Kinoshita, Shigeru (2003) Tight junction-related protein expression and distribution in human corneal epithelium. Experimental Eye Research, 76 (6). pp. 663-669.

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Abstract

Purpose. To investigate the expression and cellular distribution of the tight junction-related proteins occludin, claudin and ZO-1 in human corneal epithelium. Methods. Light and electron immunohistochemistry was used to determine tissue distribution of occludin, claudin-1 and ZO-1 in the human corneal epithelium. Reverse transcription-polymerase chain reaction was used to reveal claudin mRNA expression in human corneal epithelium. Results. In transverse sections, occludin and ZO-1 were localized at the apical cell–cell junctions between superficial cells in stratified corneal epithelium. Both basal and basolateral membranes of superficial cells were stained by the claudin-1 antibody, but no apical membrane staining was observed. In en face sections, claudin-1 and ZO-1 antibodies showed as bands that corresponded to the junctional complex. Claudin-1 staining of superficial cell cytoplasm was also observed. Occludin staining was seen at the junctional complex, where it was not continuous, but dotted along the cell junctions. The transcripts for claudin-1 and several other claudin isotypes, such as -2, -3, -4, -7, -9 and -14 were identified. Conclusion. Not only occludin, but also some claudins act as integral transmembrane proteins in the corneal epithelium. ZO-1 is a component of the corneal epithelial tight junction, as it is in most epithelial cells.

Item Type: Article
Journal or Publication Title: Experimental Eye Research
Uncontrolled Keywords: tight junction ; corneal epithelium ; occludin ; claudin ; ZO-1 ; intercellular adhesion molecules ; immunocytochemistry ; electron microscopy
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 9084
Deposited By: Dr Nigel J Fullwood
Deposited On: 23 May 2008 13:52
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:31
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9084

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