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An investigation of removed cultivated epithelial transplants in patients after allo-cultivated corneal epithelial transplantation.

Cooper, Leanne J. and Fullwood, Nigel J. and Koizumi, Noriko and Nakamura, Takahiro and Kinoshita, Shigeru (2004) An investigation of removed cultivated epithelial transplants in patients after allo-cultivated corneal epithelial transplantation. Cornea, 23 (3). pp. 235-242. ISSN 0277-3740

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Abstract

Objective: To investigate the ultrastructural changes of removed cultivated corneal epithelial transplants using scanning and transmission electron microscopy. Methods: Allocultivated corneal epithelial transplantation, using an amniotic membrane carrier, was carried out on 3 patients. The primary diagnoses consisted of 1 with acute-phase chemical burn, one with drug-induced pseudopemphigoid, and 1 with Stevens-Johnson syndrome. After a period of several months the transplants were removed from these patients because of graft opacities. The removed transplants were then prepared for examination by scanning and transmission electron microscopy. Results: In all 3 cases there was a similar pattern of findings: the amniotic membrane remained intact, although it had become partially vascularized and invaded by keratocytes. Inflammatory cells were present in the epithelial layer and within the amniotic membrane. Most of the amniotic membrane was covered by conjunctival epithelial cells and goblet cells. Only a few areas of normal cultivated corneal epithelial cells were found. Conclusions: We suggest that the process of allograft rejection is responsible for the corneal epithelial loss and that this is followed by conjunctival invasion onto the amniotic membrane.

Item Type: Article
Journal or Publication Title: Cornea
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 9077
Deposited By: Dr Nigel J Fullwood
Deposited On: 23 May 2008 13:54
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:31
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9077

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