Zhao, Bojun and Cooper, Leanne J. and Brahma, Arun and MacNeil, Sheila and Rimmer, Stephen and Fullwood, Nigel J. (2006) Development of a Three-Dimensional Organ Culture Model for Corneal Wound Healing and Corneal Transplantation. Investigative Ophthalmology & Visual Science, 47 (7). pp. 2840-2846. ISSN 0146-0404Full text not available from this repository.
PURPOSE. To develop and evaluate a three-dimensional organ culture system of the cornea anterior chamber that could replicate the in vivo processes occurring during corneal wound healing and corneal transplantation. METHODS. Bovine corneoscleral buttons were clamped in a specially designed chamber through the sclera outside the limbus. The epithelium was exposed to air, and its anterior surface was automatically irrigated. The endothelial layer was perfused separately with media under normal intraocular pressure. Wound healing and corneal transplantation were observed using light, scanning, and transmission electron microscopy. RESULTS. The organ culture system maintained the epithelium, the putative epithelial stem cells in the limbus, the stroma, and the endothelium in good condition for the 10-day period during which the system was evaluated. The authors observed that the processes of wound healing and corneal transplantation in the model appeared similar to those occurring in vivo. CONCLUSIONS. In vitro model closely replicated the in vivo processes of wound healing and corneal transplantation. The authors believe this model will be useful for basic investigations into the cornea, such as study of the response of the cornea to surgery, wound healing, toxins, and therapeutic agents.
|Journal or Publication Title:||Investigative Ophthalmology & Visual Science|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Health and Medicine > Biomedical & Life Sciences|
Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Science and Technology > Physics
|Deposited By:||Dr Nigel J Fullwood|
|Deposited On:||23 May 2008 13:54|
|Last Modified:||01 Oct 2013 11:38|
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