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Characterization of Putative Stem Cell Populations in the Cornea Using Synchrotron Infrared Microspectroscopy.

German, Matthew J. and Pollock, Hubert M. and Zhao, Bojun and Tobin, Mark J. and Hammiche, Azzedine and Bentley, Adam and Cooper, Leanne J. and Martin, Francis L. and Fullwood, Nigel J. (2006) Characterization of Putative Stem Cell Populations in the Cornea Using Synchrotron Infrared Microspectroscopy. Investigative Ophthalmology and Visual Science, 47 (6). pp. 2417-2421.

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Abstract

PURPOSE. High-resolution synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopy coupled with multivariate analysis was used to investigate the characteristics of putative adult stem cell (SC), transiently amplified (TA) cell, and terminally differentiated (TD) cell populations of the corneal epithelium. METHODS. Spectra of individual cells in situ in cryosections of bovine cornea were collected by using a synchrotron microspectroscopy facility at Daresbury Laboratory (United Kingdom). The resultant spectra were analyzed by multivariate analysis. RESULTS. The median spectra of the three different cell populations showed marked differences, which correlated with their degree of differentiation and proliferative capacity. Multivariate (principal component) analysis (PCA) showed that the three cell populations could be segregated into discrete clusters, with only a slight overlap between the SC and TA cell populations. Further analysis (Mann-Whitney test) indicated that the most significant (P < 0.001) spectral differences between the SC and TA cell populations were chiefly associated with changes in nucleic acid conformation. CONCLUSIONS. SR-FTIR microspectroscopy coupled with PCA appears to enable the identification of SC, TA cell, and TD cell populations. The results also suggest that a small subpopulation of cells in the corneal epithelial SC niche possess TA cell-like characteristics. The most significant spectral characteristics associated with the SCs appear to relate to differences in nucleic acid conformation. This finding is consistent with recent theories suggesting that the control of differentiation is related to major changes in chromatin structure.

Item Type: Article
Journal or Publication Title: Investigative Ophthalmology and Visual Science
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Health Research
Faculty of Science and Technology > Physics
Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 9066
Deposited By: Dr Nigel J Fullwood
Deposited On: 23 May 2008 13:55
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:31
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9066

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