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Infrared Spectroscopy with Multivariate Analysis Potentially Facilitates the Segregation of Different Types of Prostate Cell.

German, M. J. and Hammiche, A. and Ragavan, N. and Tobin, M. J. and Cooper, Leanne J. and Matanhelia, S. S. and Hindley, A. C. and Nicholson, C. M. and Fullwood, N. J. and Pollock, H. M. and Martin, F. L. (2006) Infrared Spectroscopy with Multivariate Analysis Potentially Facilitates the Segregation of Different Types of Prostate Cell. Biophysical Journal, 90 (10). pp. 3783-3795. ISSN 0006-3495

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    Abstract

    The prostate gland is conventionally divided into zones or regions. This morphology is of clinical significance as prostate cancer (CaP) occurs mainly in the peripheral zone (PZ). We obtained tissue sets consisting of paraffin-embedded blocks of cancer-free transition zone (TZ) and PZ and adjacent CaP from patients (n = 6) who had undergone radical retropubic prostatectomy; a seventh tissue set of snap-frozen PZ and TZ was obtained from a CaP-free gland removed after radical cystoprostatectomy. Paraffin-embedded tissue slices were sectioned (10-µm thick) and mounted on suitable windows to facilitate infrared (IR) spectra acquisition before being dewaxed and air dried; cryosections were dessicated on BaF2 windows. Spectra were collected employing synchrotron Fourier-transform infrared (FTIR) microspectroscopy in transmission mode or attenuated total reflection-FTIR (ATR) spectroscopy. Epithelial cell and stromal IR spectra were subjected to principal component analysis to determine whether wavenumber-absorbance relationships expressed as single points in "hyperspace" might on the basis of multivariate distance reveal biophysical differences between cells in situ in different tissue regions. After spectroscopic analysis, plotted clusters and their loadings curves highlighted marked variation in the spectral region containing DNA/RNA bands (1490–1000 cm–1). By interrogating the intrinsic dimensionality of IR spectra in this small cohort sample, we found that TZ epithelial cells appeared to align more closely with those of CaP while exhibiting marked structural differences compared to PZ epithelium. IR spectra of PZ stroma also suggested that these cells are structurally more different to CaP than those located in the TZ. Because the PZ exhibits a higher occurrence of CaP, other factors (e.g., hormone exposure) may modulate the growth kinetics of initiated epithelial cells in this region. The results of this pilot study surprisingly indicate that TZ epithelial cells are more likely to exhibit what may be a susceptibility-to-adenocarcinoma spectral signature. Thus, IR spectroscopy on its own may not be sufficient to identify premalignant prostate epithelial cells most likely to progress to CaP.

    Item Type: Article
    Journal or Publication Title: Biophysical Journal
    Subjects: Q Science > QH Natural history > QH301 Biology
    Departments: Faculty of Health and Medicine > Health Research
    Faculty of Health and Medicine > Medicine
    Faculty of Science and Technology > Lancaster Environment Centre
    Faculty of Health and Medicine > Biomedical & Life Sciences
    Faculty of Science and Technology > Physics
    ID Code: 9064
    Deposited By: Dr Nigel J Fullwood
    Deposited On: 23 May 2008 13:55
    Refereed?: Yes
    Published?: Published
    Last Modified: 26 Jul 2012 18:31
    Identification Number:
    URI: http://eprints.lancs.ac.uk/id/eprint/9064

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