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An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours.

Whitham, K. M. and Hadley, J. L. and Morris, H. G. and Andrew, S. M. and Nieduszynski, Ian A. and Brown, Gavin M. (1999) An improved method for the structural profiling of keratan sulphates : analysis of keratan suplhates from brain and ovarian tumours. Glycobiology, 9 (3). pp. 285-291.

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Abstract

A previously developed method for the structural fingerprinting of keratan sulfates (Brown et al., Glycobiology, 5, 311-317, 1995) has been adapted for use with oligosaccharides fluorescently labeled with 2- aminobenzoic acid following keratanase II digestion. The oligosaccharides are separated by high-pH anion-exchange chromatography on a Dionex AS4A-SC column. This methodology permits quantitative analysis of labeled oligosaccharides which can be detected at the sub- nanogram ( approximately 100 fmol) level. Satisfactory calibration of this method can be achieved using commercial keratan sulfate standards. Keratan sulfates from porcine brain phosphocan and human ovarian tumors have been examined using this methodology, and their structural features are discussed.

Item Type: Article
Journal or Publication Title: Glycobiology
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
Faculty of Health and Medicine > Health Research
Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 9062
Deposited By: Dr Gavin M Brown
Deposited On: 23 May 2008 13:56
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:31
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9062

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