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Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides.

Huckerby, Thomas N. and Lauder, Robert M. and Brown, Gavin M. and Nieduszynski, Ian A. and Anderson, Karen and Boocock, Joanne and Sandall, Patricia L. and Weeks, Stephen D. (2001) Characterisation of oligosaccharides from the chondroitin sulfates. 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides. European Journal of Biochemistry, 268 (5). pp. 1181-1189. ISSN 0014-2956

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Abstract

Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-d-galactitol (GalNAc-ol) form. These have the structures: ΔUA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S-ol, ΔUA(β1–3)GalNAc4S(β1–4)l-IdoA(α1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc4S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol, ΔUA(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc4S-ol and ΔUA2S(β1–3)GalNAc6S(β1–4)GlcA(β1–3)GalNAc6S-ol, where ΔUA represents a 4,5-unsaturated hexuronic acid (4-deoxy-α-lthreo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete 1H-NMR and 13C-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers.

Item Type: Article
Journal or Publication Title: European Journal of Biochemistry
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Biomedical & Life Sciences
VC's Office
ID Code: 9058
Deposited By: Dr Gavin M Brown
Deposited On: 23 May 2008 13:58
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:31
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9058

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