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Identification and characterisation of a RAD51 gene from Leishmania major.

McKean, P. G. and Keen, J. G. and Smith, D. F. and Benson, Fiona E. (2001) Identification and characterisation of a RAD51 gene from Leishmania major. Molecular and Biochemical Parasitology, 115 (2). pp. 209-216. ISSN 0166-6851

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Abstract

The RAD51 gene is a homologue of Escherichia coli recA which plays a central role in homologous recombination and DNA repair. This paper describes the identification of the RAD51 gene from the trypanosomatid parasite Leishmania major. The LmRAD51 gene codes for a 377 amino acid polypeptide with a predicted molecular mass of 41 259 Da that is highly homologous to the Rad51 family of proteins. Recombinant L. major Rad51 protein (LmRad51) was over-expressed in a bacterial expression system, purified to homogeneity and shown to bind DNA and exhibit DNA-stimulated ATPase activity, consistent with previously reported biochemical characteristics of Rad51 protein. Although LmRad51 expression is below the level of detection in exponentially growing cultures of Leishmania, high levels of LmRad51 mRNA and protein expression can be detected following exposure to the DNA-damaging agent phleomycin. LmRAD51 is one of the first examples of a DNA damage-inducible gene to be characterised in Leishmania, and will be invaluable in studying the contribution of homologous recombination to Leishmania virulence.

Item Type: Article
Journal or Publication Title: Molecular and Biochemical Parasitology
Uncontrolled Keywords: Rad51 ; Leishmania ; Homologous recombination ; Gene amplification
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 9054
Deposited By: Dr Fiona E Benson
Deposited On: 21 May 2008 16:22
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:31
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9054

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