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Complex formation by the human RAD51C and XRCC3 recombination repair proteins.

Masson, J. Y. and Stasiak, A. Z. and Stasiak, A. and Benson, Fiona E. and West, S. C. (2001) Complex formation by the human RAD51C and XRCC3 recombination repair proteins. Proceedings of the National Academy of Sciences, 98 (15). pp. 8440-8446. ISSN 0027-8424

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Abstract

In vertebrates, the RAD51 protein is required for genetic recombination, DNA repair, and cellular proliferation. Five paralogs of RAD51, known as RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3, have been identified and also shown to be required for recombination and genome stability. At the present time, however, very little is known about their biochemical properties or precise biological functions. As a first step toward understanding the roles of the RAD51 paralogs in recombination, the human RAD51C and XRCC3 proteins were overexpressed and purified from baculovirus-infected insect cells. The two proteins copurify as a complex, a property that reflects their endogenous association observed in HeLa cells. Purified RAD51C-XRCC3 complex binds single-stranded, but not duplex DNA, to form protein-DNA networks that have been visualized by electron microscopy.

Item Type: Article
Journal or Publication Title: Proceedings of the National Academy of Sciences
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 9045
Deposited By: Dr Fiona E Benson
Deposited On: 21 May 2008 15:26
Refereed?: Yes
Published?: Published
Last Modified: 01 Oct 2013 10:34
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/9045

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