Hunt, L. and Mills, L. N. and Pical, C. and Leckie, C. P. and Aitken, F. L. and Kopka, J. and Mueller-Roeber, B. and McAinsh, Martin and Hetherington, A. M. and Gray, J. E. (2003) Phospholiphase C is required for the control of stomatal aperture by ABA. Plant Journal, 34 (1). pp. 47-55. ISSN 0960-7412Full text not available from this repository.
The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.
|Journal or Publication Title:||Plant Journal|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
Faculty of Science and Technology > Engineering
|Deposited By:||Dr Martin R McAinsh|
|Deposited On:||20 May 2008 11:49|
|Last Modified:||06 Sep 2013 21:14|
Actions (login required)