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Bioloistic delivery of Ca2+ dyes into plant and algal cells.

Bothwell, John H. F. and Brownlee, Colin and Hetherington, Alistair M. and Ng, Carl K.-Y. and Wheeler, Glen L. and McAinsh, Martin (2006) Bioloistic delivery of Ca2+ dyes into plant and algal cells. Plant Journal, 46 (2). pp. 327-335. ISSN 0960-7412

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Abstract

In eukaryotes, changes in cytosolic Ca2+ concentrations ([Ca2+]cyt) are associated with a number of environmental and developmental stimuli. However, measuring [Ca2+]cyt changes in single plant or algal cells is often problematic. Although a wide range of Ca2+-sensitive fluorescent dyes is available, they are often difficult to introduce into plant cells. Micro-injection is the most robust method for dye loading, but is time-consuming, technically demanding, and unsuitable in many cell types. To overcome these problems, we have adapted biolistic techniques to load Ca2+-sensitive dyes into guard cells of the flowering plant, Commelina communis, cells of the green alga Chlamydomonas reinhardtii, and zygotes of the brown alga, Fucus serratus. Using this approach, we have been able to monitor [Ca2+]cyt changes in response to various stimuli, including a novel [Ca2+]cyt response in C. reinhardtii. The method allows the use of free acid and dextran-conjugated dyes. Biolistic loading of differentiated plant cells is easier, quicker, and more widely applicable than micro-injection, and should broaden the study of plant signal transduction.

Item Type: Article
Journal or Publication Title: Plant Journal
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Science and Technology > Engineering
ID Code: 8941
Deposited By: Dr Martin R McAinsh
Deposited On: 20 May 2008 11:55
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 21:13
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8941

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