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Rapid low temperature induced stomatal closure occurs in cole tolerant Commelina communis leaves but not in cold sensitive Nicotiana rutica leaves via a mechanism that involves apoplastic calcium but not abscisic acid.

Wilkinson, Sally and Clephan, Alison Lee and Davies, William John (2001) Rapid low temperature induced stomatal closure occurs in cole tolerant Commelina communis leaves but not in cold sensitive Nicotiana rutica leaves via a mechanism that involves apoplastic calcium but not abscisic acid. Plant Physiology, 126 (4). pp. 1566-1578. ISSN 0032-0889

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Abstract

Commelina communis stomata closed within 1 h of transferring intact plants from 27°C to 7°C, whereas tobacco (Nicotiana rustica) stomata did not until the leaves wilted. Abscisic acid (ABA) did not mediate cold-induced C. communis stomatal closure: At low temperatures, bulk leaf ABA did not increase; ABA did not preferentially accumulate in the epidermis; its flux into detached leaves was lower; its release from isolated epidermis was not greater; and stomata in epidermal strips were less sensitive to exogenous ABA. Stomata of both species in epidermal strips on large volumes of cold KCl failed to close unless calcium was supplied. Therefore, the following cannot be triggers for cold-induced stomatal closure in C. communis: direct effects of temperature on guard or epidermal cells, long-distance signals, and effects of temperature on photosynthesis. Low temperature increased stomatal sensitivity to external CaCl2 by 50% in C. communis but only by 20% in tobacco. C. communis stomata were 300- to 1,000-fold more sensitive to calcium at low temperature than tobacco stomata, but tobacco epidermis only released 13.6-fold more calcium into bathing solutions than C. communis. Stomata in C. communis epidermis incubated on ever-decreasing volumes of cold calcium-free KCl closed on the lowest volume (0.2 cm3) because the epidermal apoplast contained enough calcium to mediate closure if this was not over diluted. We propose that the basis of cold-induced stomatal closure exhibited by intact C. communis leaves is increased apoplastic calcium uptake by guard cells. Such responses do not occur in chill-sensitive tobacco leaves.

Item Type: Article
Journal or Publication Title: Plant Physiology
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 8897
Deposited By: Prof William J Davies
Deposited On: 16 May 2008 11:59
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:28
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8897

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