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Using infrared thermography to study the CO2 signalling pathway in Arabidopsis.

Tagliavia, C. P. P. and Holroyd, G. H. and Davies, W. J. and McAinsh, M. R. and Hetherington, Alistair (2005) Using infrared thermography to study the CO2 signalling pathway in Arabidopsis. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 141. S274. ISSN 1095-6433

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Abstract

Stomata are small pores on leaf surfaces that regulate gas exchange. When stomata are closed evapotranspiration is reduced. This means that the temperature of leaves with closed stomata is warmer than leaves with open stomata. It is possible to visualise these temperature differences with an infrared thermal imaging camera. We are currently using this approach to isolate Arabidopsis stomatal CO2 response mutants. To do this we built a Teflon covered chamber that allows plants to be imaged from the outside. Once plants are inside the chamber we recorded thermal images during a period at ambient [CO2] (360 ppm) followed by a period at elevated [CO2] (up to 1400 ppm). Results showed that leaf temperature of 25 to 35 days old wild type plants increased 1–2 -C following exposure to elevated CO2. We screened around 18,000 EMS plants and plants that did not show the characteristic response to high CO2 were selected and seed collected. We are currently characterizing and analysing these putative mutants.

Item Type: Article
Journal or Publication Title: Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology
Additional Information: Abstracts of the Annual Main Meeting of the Society for Experimental Biology, 11th-15th July 2005, Universitat Autonoma de Barcelona, Barcelona, Spain
Uncontrolled Keywords: Thermography ; CO2 ; Stomata ; Signalling ; Arabidopsis
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 8879
Deposited By: Prof William J Davies
Deposited On: 16 May 2008 13:24
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 21:12
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8879

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