Tokuda, Takahiko and Salem, Sultan A. and Allsop, David and Mizuno, Toshiki and Nakagawa, Masanori and Qureshi, Mohamed M. and Locascio, Joseph J. and Schlossmacher, Michael G. and El-Agnaf, Omar (2006) Decreased α-synuclein in cerebrospinal fluid of aged individuals and subjects with Parkinson’s disease. Biochemical and Biophysical Research Communications, 349 (1). pp. 162-166. ISSN 0006-291XFull text not available from this repository.
There is ample biochemical, pathological, and genetic evidence that the metabolism of -synuclein (-syn) plays a crucial role in the pathogenesis of Parkinson disease (PD). To examine whether quantification of -syn in cerebrospinal fluid (CSF) is potentially informative in the diagnosis of PD, we developed a specific ELISA system and measured the concentration of -syn in CSF from 33 patients with PD (diagnosed according to UK PD Society Brain Bank criteria) and 38 control subjects including 9 neurologically healthy individuals. We found that PD patients had significantly lower -syn levels in their CSF than the control groups (p < 0.0001) even after adjusting for gender and age. Age was independently associated with lower -syn levels. Logistic regression analysis showed that reduction in CSF -syn served as a significant predictor of PD beyond age and gender alone (area under ROC curve, c = 0.882). Furthermore, we observed a close inverse correlation between -syn levels in CSF and assigned Hoehn and Yahr score in this cohort of 71 living subjects (p < 0.0001), even after adjusting for age. These findings identify in the quantification of -syn from CSF a potential laboratory marker to aid the clinical diagnosis of PD.
|Journal or Publication Title:||Biochemical and Biophysical Research Communications|
|Uncontrolled Keywords:||Parkinson disease ; Neurodegenerative diseases ; -Synuclein ; Cerebrospinal fluid ; ELISA ; Biomarker ; Diagnosis|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
Faculty of Health and Medicine > Biomedical & Life Sciences
|Deposited By:||Prof David Allsop|
|Deposited On:||15 May 2008 11:47|
|Last Modified:||26 Jul 2012 18:27|
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