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A spectroscopic study of some of the peptidyl radicals formed following hydroxyl radical attack on β-amyloid and α-synuclein.

Tabner, Brian J. and Turnbull, Stuart and King, Jennifer and Benson, Fiona E. and El-Agnaf, Omar M. A. and Allsop, David (2006) A spectroscopic study of some of the peptidyl radicals formed following hydroxyl radical attack on β-amyloid and α-synuclein. Free Radical Research, 40 (7). pp. 731-739. ISSN 1071-5762

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Abstract

There is clear evidence implicating oxidative stress in the pathology of many neurodegenerative diseases. Reactive oxygen species (ROS) are the primary mediators of oxidative stress, and hydrogen peroxide, a key ROS, is generated during aggregation of the amyloid proteins associated with some of these diseases. Hydrogen peroxide is catalytically converted to the aggressive hydroxyl radical in the presence of Fe(II) and Cu(I), which renders amyloidogenic proteins such as β-amyloid and -synuclein (implicated in Alzheimer's disease (AD) and Parkinson's disease (PD), respectively) vulnerable to self-inflicted hydroxyl radical attack. Here, we report some of the peptide-derived radicals, detected by electron spin resonance spectroscopy employing sodium 3,5-dibromo-4-nitrosobenzenesulfonate as a spin-trap, following hydroxyl radical attack on Aβ(1-40), -synuclein and some other related peptides. Significantly, we found that sufficient hydrogen peroxide was self-generated during the early stages of aggregation of Aβ(1-40) to produce detectable peptidyl radicals, on addition of Fe(II). Our results support the hypothesis that oxidative damage to Aβ (and surrounding molecules) in the brain in AD could be due, at least in part, to the self-generation of ROS. A similar mechanism could operate in PD and some other “protein conformational” disorders.

Item Type: Article
Journal or Publication Title: Free Radical Research
Uncontrolled Keywords: Alzheimer's disease ; Parkinson's disease ; electron spin resonance ; reactive oxygen species ; hydroxyl radical ; hydrogen peroxide
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 8839
Deposited By: Prof David Allsop
Deposited On: 15 May 2008 11:47
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:27
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8839

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