Tinney, Glenda W. and Pritchard, Susan C. and Gonzalez, Raquel and Paul, Nigel D. and Hatcher, Paul E. and Taylor, Jane E. (2002) Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius. Plant Molecular Biology Reporter, 20 (3). pp. 309-310. ISSN 0735-9640Full text not available from this repository.
Many plant RNA isolation techniques aim to prevent contamination by means of secondary phenolics, carbohydrates, RNase, and other chemicals. However, when applied in our laboratory to the isolation of RNA fromRumex obtusifolius, these protocols failed to produce good quality RNA. A major problem was contamination of the RNA samples with the secondary metabolite oxalate. The relative quantities of guanidine isothiocyanate extraction buffer to plant tissue used in the protocol had significant effects on oxalate contamination. An increase in extraction buffer, from 1.5 mL in the original method to 15 mL per 200–300 mg of tissue in our protocol, removed the oxalate from the RNA. This RNA was of a good quality and was suitable for molecular biology applications.
|Journal or Publication Title:||Plant Molecular Biology Reporter|
|Uncontrolled Keywords:||RNA extraction - oxalate contamination - Rumex|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Dr Nigel Paul|
|Deposited On:||13 May 2008 15:25|
|Last Modified:||26 Jul 2012 18:27|
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