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Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius.

Tinney, Glenda W. and Pritchard, Susan C. and Gonzalez, Raquel and Paul, Nigel D. and Hatcher, Paul E. and Taylor, Jane E. (2002) Elimination of oxalate contamination in RNA isolation from Rumex obtusifolius. Plant Molecular Biology Reporter, 20 (3). pp. 309-310. ISSN 0735-9640

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Abstract

Many plant RNA isolation techniques aim to prevent contamination by means of secondary phenolics, carbohydrates, RNase, and other chemicals. However, when applied in our laboratory to the isolation of RNA fromRumex obtusifolius, these protocols failed to produce good quality RNA. A major problem was contamination of the RNA samples with the secondary metabolite oxalate. The relative quantities of guanidine isothiocyanate extraction buffer to plant tissue used in the protocol had significant effects on oxalate contamination. An increase in extraction buffer, from 1.5 mL in the original method to 15 mL per 200–300 mg of tissue in our protocol, removed the oxalate from the RNA. This RNA was of a good quality and was suitable for molecular biology applications.

Item Type: Article
Journal or Publication Title: Plant Molecular Biology Reporter
Uncontrolled Keywords: RNA extraction - oxalate contamination - Rumex
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 8817
Deposited By: Dr Nigel Paul
Deposited On: 13 May 2008 15:25
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 21:10
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8817

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