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A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion.

Blunt, Tracy and Taccioli, Guillermo E. and Priestley, Anne and Hafezparast, Majid and McMillan, Trevor J. and Liu, Jing and Cole, Charlotte C. and White, Jacqueline and Alt, Frederick W. and Jackson, Stephen P. and Schurr, Erwin and Lehmann, Alan R. and Jeggo, Penny A. (1995) A YAC Contig Encompassing the XRCC5 (Ku80) DNA Repair Gene and Complementation of Defective Cells by YAC Protoplast Fusion. Genomics, 30 (2). pp. 320-328. ISSN 0888-7543

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Abstract

The Chinese hamster ovaryxrsmutants are sensitive to ionizing radiation, defective in DNA double-strand break rejoining, and unable to carry out V(D)J recombination effectively. Recently, the gene defective in these mutants, XRCC5, has been shown to encode Ku80, a component of the Ku protein and DNA-dependent protein kinase. We present here a YAC contig involving 25 YACs mapping to the region 2q33–q34, which encompasses the XRCC5 gene. Eight new markers for this region of chromosome 2 are identified. YACs encoding the Ku80 gene were transferred toxrscells by protoplast fusion, and complementation of all the defective phenotypes has been obtained with two YACs. We discuss the advantages and disadvantages of this approach as a strategy for cloning human genes complementing defective rodent cell lines.

Item Type: Article
Journal or Publication Title: Genomics
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 8767
Deposited By: Professor Trevor McMillan
Deposited On: 12 May 2008 15:59
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:26
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8767

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