Lancaster EPrints

DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene.

Ross, Gillian and Eady, John J. and Mithal, Natasha P. and Bush, Cyd and Steel, G. Gordon and Jeggo, Penny A. and McMillan, Trevor J. (1995) DNA Strand Break Rejoining Defect in xrs-6 Is Complemented by Transfection with the Human Ku80 Gene. Cancer Research, 55 (6). pp. 1235-1238. ISSN 0008-5472

Full text not available from this repository.

Abstract

The radiosensitive mutant xrs-6, derived from Chinese hamster ovary cell line CHO-K1, has been demonstrated to be defective in DNA double-strand break repair and also in its proficiency to undergo V(D)J recombination. Recent work has provided both genetic and biochemical evidence that the Mr 80,000 subunit of the Ku protein is able to complement the radiosensitivity and the V(D)J recombination defect in the xrs-6 mutant. We demonstrate here that complementation of the radiosensitive phenotype in xrs-6 cells by the introduction of Ku80 cDNA is accompanied by the concomitant restoration of DNA double-strand break rejoining proficiency to almost that of the parental CHO-K1 cells, as measured both by neutral single-cell microgel electrophoresis (Comet) technique and by pulsed-field gel electrophoresis. These results provide further biochemical evidence for the involvement of the Ku protein in the repair of DNA double-strand breaks.

Item Type: Article
Journal or Publication Title: Cancer Research
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 8765
Deposited By: Professor Trevor McMillan
Deposited On: 12 May 2008 16:01
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:26
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8765

Actions (login required)

View Item