Ruiz de Almodovar, J. M. and Nunez, M. I. and McMillan, T. J. and Olea, N. and Mort, C. and Villalobos, M. and Pedraza, V. and Steel, G. G. (1994) Initial radiation-induced DNA damage in human tumour cell lines : a correlation with intrinsic radiosensitivity. British Journal of Cancer, 69 (3). pp. 457-462. ISSN 1532-1827Full text not available from this repository.
The role of the initial DNA double-strand breaks (dsb) as a determinant of cellular radiosensitivity was studied in human breast and bladder cancer cell lines. Cell survival was measured by monolayer colony-forming assay as appropriate and differences in radiosensitivity were seen (alpha-values ranged from 0.12 to 0.54). After pulsed-field gel electrophoresis (PFGE) the initial slopes of dose-response curves were biphasic with a flattening of the curves above 30 Gy. When the frequency of DNA dsb induction was assessed using a mathematical model based on the DNA fragment size distribution into the gel lane, we found a statistically significant relationship between the number of DNA dsb induced and the corresponding alpha-values and fraction surviving after 2Gy (P = 0.0049 and P = 0.0031 respectively). These results support the view that initial damage is a major determinant of cell radiosensitivity.
|Journal or Publication Title:||British Journal of Cancer|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Professor Trevor McMillan|
|Deposited On:||12 May 2008 16:04|
|Last Modified:||27 May 2016 01:12|
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