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Initial radiation-induced DNA damage in human tumour cell lines : a correlation with intrinsic radiosensitivity.

Ruiz de Almodovar, J. M. and Nunez, M. I. and McMillan, T. J. and Olea, N. and Mort, C. and Villalobos, M. and Pedraza, V. and Steel, G. G. (1994) Initial radiation-induced DNA damage in human tumour cell lines : a correlation with intrinsic radiosensitivity. British Journal of Cancer, 69 (3). pp. 457-462. ISSN 1532-1827

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Abstract

The role of the initial DNA double-strand breaks (dsb) as a determinant of cellular radiosensitivity was studied in human breast and bladder cancer cell lines. Cell survival was measured by monolayer colony-forming assay as appropriate and differences in radiosensitivity were seen (alpha-values ranged from 0.12 to 0.54). After pulsed-field gel electrophoresis (PFGE) the initial slopes of dose-response curves were biphasic with a flattening of the curves above 30 Gy. When the frequency of DNA dsb induction was assessed using a mathematical model based on the DNA fragment size distribution into the gel lane, we found a statistically significant relationship between the number of DNA dsb induced and the corresponding alpha-values and fraction surviving after 2Gy (P = 0.0049 and P = 0.0031 respectively). These results support the view that initial damage is a major determinant of cell radiosensitivity.

Item Type: Article
Journal or Publication Title: British Journal of Cancer
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 8762
Deposited By: Professor Trevor McMillan
Deposited On: 12 May 2008 16:04
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:26
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8762

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