Whitaker, Stephen J. and McMillan, Trevor J. (1992) Oxygen effect for double-strand break induction determined by pulsed-field gel electrophoresis. International Journal of Radiation Biology, 61 (1). pp. 29-41. ISSN 1362-3095Full text not available from this repository.
The induction of DNA double-strand breaks (dsb) following irradiation under oxygenated and hypoxic conditions with and without misonidazole was measured by pulsed-field gel electrophoresis (PFGE) in a human bladder carcinoma cell line. The dose-response curve for DNA dsb detection by PFGE was biphasic with an apparent reduction in rate of dsb induced with dose. Oxygen enhancement ratios (OER) for cell survival (at a surviving fraction of 0·1) and for DNA damage assessed by PFGE (at 80% retained) were 2·0 and 3·0 respectively. Dose-modifying factors for misonidazole (15 mm), of 1·9 (survival) and 2·4 (DNA damage) were found. Although the magnitude of the inter-experiment variations limit the precision with which cell survival and DNA electrophoresis can be compared, the data do support a simple correlation between these two measures of response. When DNA dsb induction frequency was assessed from the number average molecular weight, values of 2·7 (±0·3), 0·7 (±0·1) and 2·6 (±0·5) × 10-9 dsb/bp/Gy were found for irradiation under oxic, hypoxic alone and hypoxic + misonidazole conditions respectively. This gives an OER of 3·9 and a DMF of 3·7.
|Journal or Publication Title:||International Journal of Radiation Biology|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Departments:||Faculty of Science and Technology > Lancaster Environment Centre|
|Deposited By:||Professor Trevor McMillan|
|Deposited On:||12 May 2008 16:08|
|Last Modified:||26 Jul 2012 18:26|
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