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Molecular assays of radiation-induced DNA damage.

Whitaker, Stephen J. and Powell, Simon N. and McMillan, Trevor J. (1991) Molecular assays of radiation-induced DNA damage. European Journal of Cancer and Clinical Oncology, 27 (7). pp. 922-928. ISSN 0277-5379

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Abstract

There is a need for assays of DNA damage in many areas of laboratory research applied to radiation therapy, in order to understand the molecular processes involved in cell killing by ionising radiation and to predict in vivo response. Assays exist which measure many types of DNA damage following ionising radiation. From studies of the dose-response relationships for different types of damage, the double-strand break (dsb) has been shown to be the most significant lesion. Assays for DNA dsb have been of low sensitivity, such that supralethal doses of radiation had to be used in order to study dsb induction or repair. New assays, such as pulsed-field gel electrophoresis, are sensitive to dsb in a dose range relevant to cell survival. In addition, these assays can assess the distribution of dsb in different parts of the genome and determine heterogeneity of damage induction and repair. Assays which measure the effects of strand breaks on DNA complexed with nuclear matrix can reveal features of chromatin organisation and their influence on cellular radiosensitivity.

Item Type: Article
Journal or Publication Title: European Journal of Cancer and Clinical Oncology
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 8737
Deposited By: Professor Trevor McMillan
Deposited On: 12 May 2008 16:10
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:26
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8737

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