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The use of tetrazolium assay in measuring the response of human tumour cells to ionising radiation.

Price, Patricia and McMillan, Trevor J. (1990) The use of tetrazolium assay in measuring the response of human tumour cells to ionising radiation. Cancer Research, 50 (5). pp. 1392-1396. ISSN 0008-5472

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Abstract

Three human tumor cell lines of widely differing radiosensitivity were used to examine the characteristics of the 3-[4,5-dimethyl(thiazol-2-yl)-3,5-diphery]tetradium bromide (MTT) assay and to select suitable conditions for its use in assessing the response of cells to ionizing radiation. The optimal concentration of MTT and the time of incubation of the cells with MTT were individualized for each cell line. The relationship between absorbance and cell number was not linear over the wide range of cell numbers that were used. A calibration curve of absorbance against cell number for each cell line was therefore used. Using the assay to quantify metabolically viable cells, growth curves of irradiated and unirradiated cells were constructed on days 0–14 after irradiation. Accurate surviving fractions could be calculated only when cells were in exponential growth. Using this modification to its interpretation, the MTT assay was able to provide a reproducible measure of survival, which compared well with clonogenic cell survival measurements. However, the necessity to optimize conditions of the MTT assay for each cell line severely limits its usefulness in determining the radiosensitivity of cells in primary human tumor cultures.

Item Type: Article
Journal or Publication Title: Cancer Research
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 8734
Deposited By: Professor Trevor McMillan
Deposited On: 12 May 2008 16:11
Refereed?: Yes
Published?: Published
Last Modified: 26 Jul 2012 18:25
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8734

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