Lancaster EPrints

Role for non-homologous end-joining in the repair of UVA induced damage.

Fell, L. J. and Paul, N. D. and McMillan, T. J. (2002) Role for non-homologous end-joining in the repair of UVA induced damage. International Journal of Radiation Biology, 78 (11). pp. 1023-1027. ISSN 1362-3095

Full text not available from this repository.

Abstract

Purpose : The biological significance of long-wavelength ultraviolet (UV) light, UVA, is increasingly realized, but the precise nature of the cellular damage responsible for the effects of this radiation is still not clear. It has been reported that UVA can induce double-strand breaks in DNA, but the biological significance of these is not known. We have therefore examined the UVA sensitivity of a cell line deficient in non-homologous end-joining, the major pathway for the repair of DNA double-strand breaks in mammalian cells in order to determine the biological importance of UVA-induced DSB. Materials and methods : Xrs-6, a Chinese hamster ovary cell line mutant for XRCC5 (Ku80) was compared with its parental CHO-K1 cell line for its sensitivity to UVA radiation (365 nm) using both a clonogenic assay and the micronucleus assay. Results : Xrs-6 cells were sensitive to the cytotoxic effects of UVA. This resulted in the formation of chromosome damage, as measured by the micronucleus assay, which this cell line was unable to repair. Conclusions : Owing to the nature of the repair defect in these cells, these results imply that DNA double-strand breaks are produced in cells following UVA irradiation, that the non-homologous end-joining repair pathway is involved in their repair and that they are produced with sufficient frequency to have biological significance.

Item Type: Article
Journal or Publication Title: International Journal of Radiation Biology
Subjects: Q Science > QH Natural history > QH301 Biology
Departments: Faculty of Science and Technology > Lancaster Environment Centre
VC's Office
ID Code: 8714
Deposited By: Professor Trevor McMillan
Deposited On: 09 May 2008 10:17
Refereed?: Yes
Published?: Published
Last Modified: 06 Sep 2013 21:08
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8714

Actions (login required)

View Item