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Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei

Robinson, Nicholas P. and McCulloch, Richard and Conway, Colin and Browitt, Alison and Barry, J. David (2002) Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei. Journal of Biological Chemistry, 277 (29). pp. 26185-26193. ISSN 0021-9258

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Abstract

We demonstrate, by gene deletion analysis, that Mre11 has a critical role in maintaining genomic integrity in Trypanosoma brucei. mre11(-/-) null mutant strains exhibited retarded growth but no delay or disruption of cell cycle progression. They showed also a weak hyporecombination phenotype and the accumulation of gross chromosomal rearrangements, which did not involve sequence translocation, telomere loss, or formation of new telomeres. The trypanosome mre11(-/-) strains were hypersensitive to phleomycin, a mutagen causing DNA double strand breaks (DSBs) but, in contrast to mre11(-/-) null mutants in other organisms and T. brucei rad51(-/-) null mutants, displayed no hypersensitivity to methyl methanesulfonate, which causes point mutations and DSBs. Mre11 therefore is important for the repair of chromosomal damage and DSBs in trypanosomes, although in this organism the intersection of repair pathways appears to differ from that in other organisms. Mre11 inactivation appears not to affect VSG gene switching during antigenic variation of a laboratory strain, which is perhaps surprising given the importance of homologous recombination during this process.

Item Type: Journal Article
Journal or Publication Title: Journal of Biological Chemistry
Subjects: ?? AMINO ACID SEQUENCEANIMALSARABIDOPSISDNA DAMAGEDNA REPAIRENDODEOXYRIBONUCLEASESEXODEOXYRIBONUCLEASESGENE DUPLICATIONHUMANSMETHYL METHANESULFONATEPHENOTYPEPHLEOMYCINSPOINT MUTATIONSACCHAROMYCES CEREVISIAE PROTEINSSEQUENCE ALIGNMENTTRYPANOSOMA BRUCEI BRUCEI ??
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 86278
Deposited By: ep_importer_pure
Deposited On: 13 May 2017 03:34
Refereed?: Yes
Published?: Published
Last Modified: 12 Feb 2019 02:19
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/86278

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