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Immunofluorescence confocal laser scanning microscopy and immuno-electron microscopic identification of keratins in human maternofetal interaction zone.

Ahenkorah, John and Hottor, Bismarck and Byrne, Simon and Bosio, Paul and Ockleford, Colin D. (2009) Immunofluorescence confocal laser scanning microscopy and immuno-electron microscopic identification of keratins in human maternofetal interaction zone. Journal of Cellular and Molecular Medicine, 13 (4). pp. 735-748. ISSN 1582-4934

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Abstract

We show here that at least 5 keratin proteins are present in villous trophoblast and the same 5 in extravillous trophoblast. A further 14 tested were undetectable in these tissues. In contrast 10 of the 19 keratins tested were present in amniotic epithelium. The potential for marking amniotic epithelium on the one hand, as distinct from villous and extravillous trophoblast on the other can be achieved using 5 keratins (K4, 6, 13, 14 and 17) with a mixture of positive and negative discrimination that is expected, in combination, to be highly sensitive. All the specific keratins identified in trophoblast were apparently upregulated on the pathway to extravillous trophoblast. Co-ordinated differentiation at the molecular expression level is indicated by this finding. The relevant keratins are K5, 7, 8, 18 and19. Specific keratins have been identified that are down regulated in villous trophoblast in pre-eclamptic pregnancy. This difference between healthy and pre-eclamptic chorionic villous trophoblast keratin expression was statistically significant in 4 out of the 5 keratins. This was not the case for the extravillous trophoblast at the immunofluorescence confocal level but clear significant differences were obtained using immunogold electron microscopy. We suggest that the villous trophoblast in pre-eclamptic placentae is cytoskeletally weaker with respect to the filaments made from these specific proteins and that this is one reason why, in pre-eclampsia, trophoblast is deported in greater quantity than in healthy placentae.

Item Type: Article
Journal or Publication Title: Journal of Cellular and Molecular Medicine
Uncontrolled Keywords: keratin immunofluorescence • materno-foetal interaction • chorionic villi • pre-eclampsia
Subjects: Q Science > QM Human anatomy
Departments: Faculty of Health and Medicine > Medicine
ID Code: 8421
Deposited By: Professor Colin D. Ockleford
Deposited On: 23 Apr 2008 16:21
Refereed?: Yes
Published?: Published
Last Modified: 06 Aug 2013 05:30
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/8421

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