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Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device

Baldock, S. J. and Fielden, P. R. and Goddard, N. J. and Kretschmer, H. R. and Prest, J. E. and Brown, B. J. T. (2004) Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device. Journal of Chromatography A, 1042 (1-2). pp. 181-188. ISSN 0021-9673

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Abstract

A microdevice design furnished with a novel sample injector, capable of delivering variable volume samples, for miniaturised isotachophoretic separations is presented. Micromachining by direct milling was used to realise two flow channel network designs on poly(methyl methacrylate) chips. Both designs comprised a wide bore sample channel interfaced, via a short connection channel, to a narrow bore separation channel. Superior injection performance was observed with a connection channel angled at 45° to the separation channel compared to a device using a channel angled at 90°. Automated delivery of electrolytes to the microdevice was demonstrated with both hydrostatic pumping and syringe pumps; both gave reproducible sample injection. A range of different sampling strategies were investigated. Isotachophoretic separations of model analytes (metal ions and an anionic dye) demonstrated the potential of the device. Separations of ten metal cations were achieved in under 475 s.

Item Type: Article
Journal or Publication Title: Journal of Chromatography A
Uncontrolled Keywords: Isotachphoresis ; Miniaturization ; Injection methods ; Instrumentation ; Chip technology
Subjects:
Departments:
ID Code: 77395
Deposited By: ep_importer_pure
Deposited On: 23 Dec 2015 13:38
Refereed?: Yes
Published?: Published
Last Modified: 22 Nov 2017 23:38
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/77395

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