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A point mutation in the gene encoding the Rubisco large subunit interferes with holoenzyme assembly

Shikanai, Toshiharu and Foyer, Christine H. and Dulieu, Hubert and Parry, Martin A. J. and Yokota, Akiho (1996) A point mutation in the gene encoding the Rubisco large subunit interferes with holoenzyme assembly. Plant Molecular Biology, 31 (2). pp. 399-403. ISSN 0167-4412

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Abstract

Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), a key enzyme of photosynthetic CO2 fixation, is composed of 8 large and 8 small subunits. The Rubisco-deficient Nicotiana tabacum mutant Sp25 is able to synthesize the peptides for both subunits but does not contain any active holoenzyme. The phenotype is maternally inherited and thus caused by a mutation in the chloroplast genome, which also encodes the Rubisco large subunit. A comparison of the nucleotide sequences of the large subunit gene of the Sp25 mutant with that of the wild-type tobacco revealed a single nucleotide change in the Sp25 mutant. This resulted in an amino acid substitution at Gly-322, which was replaced by serine.

Item Type: Journal Article
Journal or Publication Title: Plant Molecular Biology
Uncontrolled Keywords: assembly ; chloroplast ; mutation ; Rubisco ; tobacco
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 76041
Deposited By: ep_importer_pure
Deposited On: 21 Oct 2015 06:04
Refereed?: Yes
Published?: Published
Last Modified: 22 Feb 2018 06:51
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/76041

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