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2-Carboxyarabinitol 1-phosphate (CA1P) formation through a phosphate exchange reaction catalysed by the CA1P phosphatase from French bean (Phaseolus vulgaris L.).

Andralojc, P. John and Ryan, Jane E. and Parry, M. A. J. and Keys, Alfred J. (1996) 2-Carboxyarabinitol 1-phosphate (CA1P) formation through a phosphate exchange reaction catalysed by the CA1P phosphatase from French bean (Phaseolus vulgaris L.). The Biochemical journal, 316 (2). pp. 389-393. ISSN 0264-6021

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Abstract

Transfer of the phosphate group of 2-carboxy-D-arabinitol 1-phosphate (CA1P) to 14C-labelled 2-carboxy-D-arabinitol (CA) was catalysed by extracts from leaves of Phaseolus vulgaris. This phosphotransferase activity co-purified with CA1P phosphatase, described previously. This activity was increased, up to 16-fold, by addition of bicarbonate ions at pH 9-10, suggesting rate enhancement by enzyme carbamylation. A Vmax of 1.5 μmol/min per mg of protein and a Km (for CA) of 1.8 mM were estimated for the exchange reaction, with the purified phosphatase. 2-Carboxy-D-arabinitol 1,5-bisphosphate and 2-carboxy-D-ribitol 1,5-bisphosphate, but not D-ribulose 1,5-bisphosphate, could replace CA1P as phosphate donor to [14C]CA.

Item Type: Article
Journal or Publication Title: The Biochemical journal
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 76040
Deposited By: ep_importer_pure
Deposited On: 21 Oct 2015 06:04
Refereed?: Yes
Published?: Published
Last Modified: 21 Sep 2017 01:15
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/76040

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