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Separation of the branch chain hexose, hamamelose, by high-performance liquid chromatography, and amperometric detection of hamamelose and related compounds

Andralojc, P. John and Keys, Alfred J. and Adam, Alex and Parry, M. A J (1998) Separation of the branch chain hexose, hamamelose, by high-performance liquid chromatography, and amperometric detection of hamamelose and related compounds. Journal of Chromatography A, 814 (1-2). pp. 105-110. ISSN 0021-9673

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Abstract

The identification and purification of the branch chain hexose hamamelose (2-hydroxymethyl-d-ribose) from plant leaves is described. The procedure includes metabolite extraction in acid and a series of isocratic HPLC separations. Amperometric detection of hamamelose is shown to be quantitative, having a detection limit below 1 nmol. Similar detection of the related compound, 2-carboxy-d-arabinitol, and its monophosphate ester, yields unusually small peaks. Copyright (C) 1998 Elsevier Science B.V.

Item Type: Article
Journal or Publication Title: Journal of Chromatography A
Uncontrolled Keywords: Carboxyarabinitol ; Enzymes ; Hamamelose ; Plant materials ; Rubisco ; Sugars
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 76034
Deposited By: ep_importer_pure
Deposited On: 21 Oct 2015 06:04
Refereed?: Yes
Published?: Published
Last Modified: 24 Nov 2017 06:22
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/76034

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