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Age-dependent transformation frequency in elite wheat varieties

Pastori, G. M. and Wilkinson, M. D. and Steele, S. H. and Sparks, C. A. and Jones, H. D. and Parry, M. A J (2001) Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany, 52 (357). pp. 857-863. ISSN 0022-0957

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Abstract

Wheat is a major world crop and as such is a primary target for improvement of agronomic characteristics via genetic engineering. Optimization of transformation is essential in order to overcome the relatively low transformation frequencies encountered with wheat. Transformation of elite wheat varieties is not always successful due to variability in regeneration and transformation frequencies between varieties. In this work, two elite wheat varieties with a relatively high embryogenic capacity were transformed by particle bombardment. A strong correlation between transformation frequency and the age of wheat donor plants was observed in both varieties. The mean transformation frequency rose from 0.7% to 5% when using immature embryos from old and young donor plants, respectively. This was observed in both varieties, the best bombardments achieving up to 7.3% frequency. Using explants at an optimal developmental stage from donor plants grown under environmentally-controlled conditions has improved the reproducibility of transformation efficiency of elite wheat varieties and leads to the production of apparently phenotypicaily normal, fertile, transgenic plants.

Item Type: Journal Article
Journal or Publication Title: Journal of Experimental Botany
Uncontrolled Keywords: /dk/atira/pure/subjectarea/asjc/1100/1110
Subjects: ?? BAR GENEELITE WHEAT VARIETIESTRANSFORMATION FREQUENCYUIDA GENEWHEAT TRANSFORMATIONPLANT SCIENCE ??
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine
Faculty of Health and Medicine > Medicine
ID Code: 76025
Deposited By: ep_importer_pure
Deposited On: 21 Oct 2015 06:03
Refereed?: Yes
Published?: Published
Last Modified: 29 Apr 2019 15:59
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/76025

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