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Elucidating the biosynthesis of 2-carboxyarabinitol 1-phosphate through reduced expression of chloroplastic fructose 1,6-bisphosphate phosphatase and radiotracer studies with 14CO2

Andralojc, P. John and Keys, Alfred J. and Kossmann, Jens and Parry, Martin A J (2002) Elucidating the biosynthesis of 2-carboxyarabinitol 1-phosphate through reduced expression of chloroplastic fructose 1,6-bisphosphate phosphatase and radiotracer studies with 14CO2. Proceedings of the National Academy of Sciences, 99 (7). pp. 4742-4747. ISSN 0027-8424

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Abstract

2-Carboxyarabinitol 1-phosphate limits photosynthetic CO2 assimilation at low light because it is a potent, naturally occurring inhibitor of ribulose 1,5-bisphosphate carboxylase/oxygenase. Evidence is presented that this inhibitor is derived from chloroplastic fructose 1,6-bisphosphate. First, transgenic plants containing decreased amounts of chloroplastic fructose 1,6-bisphosphate phosphatase contained increased amounts of fructose 1,6-bisphosphate and 2-carboxyarabinitol 1-phosphate and greatly increased amounts of the putative intermediates hamamelose and 2-carboxyarabinitol, which in some cases were as abundant as sucrose. Second, French bean leaves in the light were shown to incorporate 14C from 14CO2 sequentially into fructose 1,6-bisphosphate, hamamelose bisphosphate, hamamelose monophosphate, hamamelose, and 2-carboxyarabinitol. As shown previously, 14C assimilated by photosynthesis was also incorporated into 2-carboxyarabinitol 1-phosphate during subsequent darkness.

Item Type: Article
Journal or Publication Title: Proceedings of the National Academy of Sciences
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
ID Code: 76023
Deposited By: ep_importer_pure
Deposited On: 21 Oct 2015 06:03
Refereed?: Yes
Published?: Published
Last Modified: 24 Nov 2017 06:22
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/76023

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