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pH-induced conformational transitions in alpha-lactalbumin investigated with two-dimensional Raman correlation variance plots and moving windows

Ashton, Lorna and Blanch, Ewan W. (2010) pH-induced conformational transitions in alpha-lactalbumin investigated with two-dimensional Raman correlation variance plots and moving windows. Journal of Molecular Structure, 974 (1-3). pp. 132-138. ISSN 0022-2860

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Abstract

Raman spectroscopy is being increasingly applied to the investigation of conformational transitions in polypeptides and proteins, and in particular protein unfolding, due to its ability to monitor changes in secondary structure. Here we focus on the application of two different two-dimensional correlation methods, autocorrelation and moving windows techniques, to investigate the pH-induced conformational transitions in alpha-lactalbumin monitored with Raman scattering. From our results we have identified three distinct stages in the conformational transition occurring at ranges similar to pH 6.5-4.6, similar to pH 4.6-3.6 and similar to pH 3.6-1.8, suggesting the existence of three possible conformational species. The first two transition phases the conformational changes are predominantly occurring in the backbone secondary structure, while in the final transition phase changes are occurring in both secondary structure and side chain residue solvent exposure. (C) 2010 Elsevier B.V. All rights reserved.

Item Type: Article
Journal or Publication Title: Journal of Molecular Structure
Uncontrolled Keywords: 2D correlation ; Raman ; Moving windows ; Protein ; alpha-Lactalbumin ; MOLTEN GLOBULE STATE ; UV RESONANCE RAMAN ; INFRARED CORRELATION SPECTROSCOPY ; OPTICAL-ACTIVITY ; STRUCTURAL-CHARACTERIZATION ; SECONDARY STRUCTURE ; POLY(L-GLUTAMIC ACID) ; CORRELATION SPECTRA ; BETA-LACTOGLOBULIN ; PHASE-TRANSITIONS
Subjects:
Departments:
ID Code: 71937
Deposited By: ep_importer_pure
Deposited On: 28 Nov 2014 12:53
Refereed?: Yes
Published?: Published
Last Modified: 21 Nov 2017 18:57
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/71937

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