Monitoring the glycosylation status of proteins using Raman spectroscopy

Brewster, Victoria L. and Ashton, Lorna and Goodacre, Royston (2011) Monitoring the glycosylation status of proteins using Raman spectroscopy. Analytical Chemistry, 83 (15). pp. 6074-6081. ISSN 0003-2700

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Abstract

Protein-based biopharmaceuticals are becoming increasingly widely used as therapeutic agents, and the characterization of these biopharmaceuticals poses a significant analytical challenge. In particular, monitoring posttranslational modifications (PTMs), such as glycosylation, is an important aspect of this characterization because these glycans can strongly affect the stability, immunogenicity, and pharmacolcinetics of these biotherapeutic drugs. Raman spectroscopy is a powerful tool, with many emerging applications in the bioprocessing arena. Although the technique has a relatively rich history in protein science, only recently has Raman spectroscopy been investigated for assessing posttranslational modifications, including phosphorylation, acetylation, trimethylation, and ubiquitination. In this investigation, we develop for the first time Raman spectroscopy combined with multivariate data analyses, including principal components analysis and partial least-squares regression, for the determination of the glycosylation status of proteins and quantifying the relative concentrations of the native ribonuclease (RNase) A protein and RNase B glycoprotein within mixtures.

Item Type:
Journal Article
Journal or Publication Title:
Analytical Chemistry
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1600/1602
Subjects:
?? OPTICAL-ACTIVITYTRIFLUOROMETHANESULFONIC ACID2-DIMENSIONAL RAMANLINKED GLYCANSDEGLYCOSYLATIONGLYCOPROTEINSQUANTIFICATIONIDENTIFICATIONCONFORMATIONPOLYPEPTIDEANALYTICAL CHEMISTRY ??
ID Code:
71935
Deposited By:
Deposited On:
28 Nov 2014 13:00
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Sep 2023 01:19