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Isolating stem cells in the inter-follicular epidermis employing synchrotron radiation-based Fourier-transform infrared microspectroscopy and focal plane array imaging

Patel, Imran I. and Harrison, Wesley J. and Kerns, Jemma G. and Filik, Jacob and Wehbe, Katia and Carmichael, Paul L. and Scott, Andrew D. and Philpott, Mike P. and Frogley, Mark D. and Cinque, Gianfelice and Martin, Francis L. (2012) Isolating stem cells in the inter-follicular epidermis employing synchrotron radiation-based Fourier-transform infrared microspectroscopy and focal plane array imaging. Analytical and Bioanalytical Chemistry, 404 (6-7). pp. 1745-1758. ISSN 1618-2642

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Abstract

Normal function and physiology of the epidermis is maintained by the regenerative capacity of this tissue via adult stem cells (SCs). However, definitive identifying markers for SCs remain elusive. Infrared (IR) spectroscopy exploits the ability of cellular biomolecules to absorb in the mid-IR region (λ = 2.5-25 μm), detecting vibrational transitions of chemical bonds. In this study, we exploited the cell's inherent biochemical composition to discriminate SCs of the inter-follicular skin epidermis based on IR-derived markers. Paraffin-embedded samples of human scalp skin (n = 4) were obtained, and 10-μm thick sections were mounted for IR spectroscopy. Samples were interrogated in transmission mode using synchrotron radiation-based Fourier-transform IR (FTIR) microspectroscopy (15 × 15 μm) and also imaged employing globar-source FTIR focal plane array (FPA) imaging (5.4 × 5.4 μm). Dependent on the location of derived spectra, wavenumber-absorbance/intensity relationships were examined using unsupervised principal component analysis. This approach showed clear separation and spectral differences dependent on cell type. Spectral biomarkers concurrently associated with segregation of SCs, transit-amplifying cells and terminally-differentiated cells of epidermis were primarily PO(2)(-) vibrational modes (1,225 and 1,080 cm(-1)), related to DNA conformational alterations. FPA imaging coupled with hierarchical cluster analysis also indicated the presence of specific basal layer cells potentially originating from the follicular bulge, suggested by co-clustering of spectra. This study highlights PO (2) (-) vibrational modes as potential putative SC markers.

Item Type: Journal Article
Journal or Publication Title: Analytical and Bioanalytical Chemistry
Uncontrolled Keywords: Biological Markers ; Cell Differentiation ; Epidermis ; Hair Follicle ; Humans ; Molecular Imaging ; Spectroscopy, Fourier Transform Infrared ; Stem Cells
Subjects:
Departments: Faculty of Science and Technology > Lancaster Environment Centre
Faculty of Health and Medicine > Medicine
Faculty of Science and Technology > School of Computing & Communications
ID Code: 70133
Deposited By: ep_importer_pure
Deposited On: 29 Jul 2014 09:37
Refereed?: Yes
Published?: Published
Last Modified: 16 Oct 2018 04:45
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/70133

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