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Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape

Andre, Jane and King, R. Andrew and Sturzenbaum, Stephen R. and Kille, Peter and Hodson, Mark E. and Morgan, A. John (2010) Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape. Environmental Pollution, 158 (3). pp. 883-890. ISSN 0269-7491

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Abstract

A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages (‘A’ and ‘B’), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central ‘ecological island’ site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies.

Item Type: Article
Journal or Publication Title: Environmental Pollution
Uncontrolled Keywords: Ecotoxicology ; Pb & Ca ; Genotyping ; FTIR ; cDNA-libraries (SERCA)
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 67955
Deposited By: ep_importer_pure
Deposited On: 11 Dec 2013 09:20
Refereed?: Yes
Published?: Published
Last Modified: 21 Nov 2017 18:44
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/67955

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