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Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore

Urbaniak, Michael D. and Muskett, Frederick W. and Finucane, Michael D. and Caddick, Stephen and Woolfson, Derek N. (2002) Solution structure of a novel chromoprotein derived from apo-neocarzinostatin and a synthetic chromophore. Biochemistry, 41 (39). pp. 11731-11739. ISSN 0006-2960

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Abstract

The natural complex Neocarzinostatin comprises a labile chromophore noncovalently bound to an 11.2 kDa protein. We present the first high-resolution structure of a novel complex derived from the recombinant apoprotein bound to a non-natural synthetic chromophore. Fluorescence and nuclear magnetic resonance spectroscopy were used to probe the strength and location of binding. Binding occurred in a location similar to that observed for the chromophore in the natural Neocarzinostatin complex, but with a distinct orientation. These results provide structural evidence that the apoprotein can readily accommodate small druglike entities, other than the natural chromophore within its binding cleft. The clinical use of the natural complex described by others, together with the results reported here, suggests potential applications for small molecule binding by apo-Neocarzinostatin.

Item Type: Journal Article
Journal or Publication Title: Biochemistry
Uncontrolled Keywords: Antibiotics, Antineoplastic ; Apoproteins ; Binding Sites ; Circular Dichroism ; Crystallography, X-Ray ; Enediynes ; Nuclear Magnetic Resonance, Biomolecular ; Recombinant Fusion Proteins ; Solutions ; Spectrometry, Fluorescence ; Zinostatin
Subjects:
Departments: Faculty of Health and Medicine > Biomedical & Life Sciences
ID Code: 66402
Deposited By: ep_importer_pure
Deposited On: 17 Sep 2013 09:09
Refereed?: Yes
Published?: Published
Last Modified: 18 Sep 2018 04:11
Identification Number:
URI: http://eprints.lancs.ac.uk/id/eprint/66402

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