Amyloid-derived peptide forms self-assembled monolayers on gold nanoparticle with a curvature-dependent β-sheet structure

Shaw, Christopher P and Middleton, David A and Volk, Martin and Lévy, Raphaël (2012) Amyloid-derived peptide forms self-assembled monolayers on gold nanoparticle with a curvature-dependent β-sheet structure. ACS Nano, 6 (2). pp. 1416-26. ISSN 1936-0851

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Abstract

Using a combination of Fourier transform infrared (FTIR) spectroscopy and solid-state nuclear magnetic resonance (SSNMR) techniques, the secondary structure of peptides anchored on gold nanoparticles of different sizes is investigated. The structure of the well-studied CALNN-capped nanoparticles is compared to the structure of nanoparticles capped with a new cysteine-terminated peptide, CFGAILSS. The design of that peptide is derived from the minimal amyloidogenic sequence FGAIL of the human islet polypeptide amylin. We demonstrate that CFGAILSS forms extended fibrils in solution. When constrained at a nanoparticle surface, CFGAILSS adopts a secondary structure markedly different from CALNN. Taking into account the surface selection rules, the FTIR spectra of CFGAILSS-capped gold nanoparticles indicate the formation of β-sheets which are more prominent for 25 nm diameter nanoparticles than for 5 nm nanoparticles. No intermolecular (13)C-(13)C dipolar coupling is detected with rotational resonance SSNMR for CALNN-capped nanoparticles, while CALNN is in a random coil configuration. Coupling is detected for CFGAILSS-capped gold nanoparticles, however, consistent with an intermolecular (13)C-(13)C distance of 5.0 ± 0.3 Å, in agreement with intermolecular hydrogen bonding in a parallel β-sheet structure.

Item Type:
Journal Article
Journal or Publication Title:
ACS Nano
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2200
Subjects:
?? AMINO ACID SEQUENCEAMYLOIDGOLDHYDROGEN BONDINGMAGNETIC RESONANCE SPECTROSCOPYMETAL NANOPARTICLESPARTICLE SIZEPEPTIDE FRAGMENTSPROTEIN STRUCTURE, SECONDARYSPECTROSCOPY, FOURIER TRANSFORM INFRAREDWATERPHYSICS AND ASTRONOMY(ALL)MATERIALS SCIENCE(ALL)ENGINEER ??
ID Code:
66258
Deposited By:
Deposited On:
17 Sep 2013 08:03
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Sep 2023 01:08